Transfer of the waterfall source isolate Pectobacterium carotovorum M022 to Pectobacterium fontis sp. nov., a deep-branching species within the genus Pectobacterium

Said Oulghazi , Jeremy Cigna (1, 2, 3, 4) , Yin Yin Lau , Mohieddine Moumni (5) , Kok Gan Chan , Denis Faure (2, 4, 3)

1 UMR ECOFOG - Ecologie des forêts de Guyane
2 I2BC - Institut de Biologie Intégrative de la Cellule
3 PBI - Intéractions Plantes-Bactéries
4 Dpt Microbio - Département Microbiologie
5 Fac Sci, Dept Biol

Pectobacterium carotovorum M022(T) has been isolated from a waterfall source in Selangor district (Malaysia). Using genomic and phenotypic tests, we re-examined the taxonomical position of this strain. Based on 14 concatenated housekeeping genes (fusA, rpoD, rpoS, acnA, purA, gyrB, recA, mdh, mtID, groEL, secY, glyA, gapA and rpIB), multi-locus sequence analysis revealed that strain M022(T) falls into a novel Glade separated from the other Pectobacterium species. The in silico DNA-DNA hybridization and average nucleotide identity values were lower than the 70 and 95% threshold values, respectively. In addition, by combining genomic and phenotypic tests, strain M022(T) may be distinguished from the other Pectobacterium isolates by its incapacity to grow on D(+)-xylose, L-rhamnose, cellobiose and lactose. Strain M022(T) (=CFBP 8629(T)=LMG 30744(T)) is proposed as the type strain of the Pectobacterium fontis sp. nov.

Mots clés: Pectobacterium; 1st report; atrosepticum; bacterial soft-rot; brasiliense; Dickeya; pathogen; pectinolytic; potato; stem rot; subsp carotovorum; taxonomy; wasabiae; water; waterfall

Domaines: Sciences du Vivant [q-bio]

Said Oulghazi, Jeremy Cigna, Yin Yin Lau, Mohieddine Moumni, Kok Gan Chan, et al.. Transfer of the waterfall source isolate Pectobacterium carotovorum M022 to Pectobacterium fontis sp. nov., a deep-branching species within the genus Pectobacterium. International Journal of Systematic and Evolutionary Microbiology, 2019, 69 (2), pp.470--475. ⟨10.1099/ijsem.0.003180⟩. ⟨hal-02173497⟩