Subterranean Clover Stunt Virus Revisited: Detection of Two Missing Genome Components

Dennis Knierim (1) , Quentin Barrière (2, 3, 4) , Ioana Grigoras (5) , Stephan Winter (1) , Heinrich-Josef Vetten , Mark Schwinghamer (6) , John Thomas (7) , Paul Chu (8) , Bruno Gronenborn (9) , Tatiana Timchenko (9)

1 Leibniz-Institut Deutsche Sammlung von Mikroorganismen und Zelllkulturen GmBH - DSMZ (GERMANY)
2 Dpt Microbio - Département Microbiologie
3 PBI - Intéractions Plantes-Bactéries
4 PROMTI - Maturation des proteines, destinée cellulaire et thérapeutique
5 ISV - Institut des sciences du végétal
6 Tamworth Agricultural Institute
7 QAAFI - Queensland Alliance for Agriculture and Food Innovation
8 Institute of Microbiology
9 I2BC - Institut de Biologie Intégrative de la Cellule

Subterranean clover stunt virus (SCSV) is a type species of the genus Nanovirus in the family Nanoviridae. It was the first single-stranded DNA plant virus with a multipartite genome, of which genomic DNA sequences had been determined. All nanoviruses have eight genome components except SCSV, for which homologs of two genome components present in all other nanovirus genomes, DNA-U2 and DNA-U4, were lacking. We analysed archived and more recent samples from SCSV-infected legume plants to verify its genome composition and found the missing genome components. These results indicated that SCSV also has eight genome components and is a typical member of the genus Nanovirus.

Mots clés: nanovirus; virus evolution; rolling circle replication; high-throughput sequencing

Domaines: Virologie; Génomique, Transcriptomique et Protéomique [q-bio.GN]

Dennis Knierim, Quentin Barrière, Ioana Grigoras, Stephan Winter, Heinrich-Josef Vetten, et al.. Subterranean Clover Stunt Virus Revisited: Detection of Two Missing Genome Components. Viruses, 2019, 11 (2), pp.138. ⟨10.3390/v11020138⟩. ⟨hal-04412732⟩