Auto-degradable and biocompatible superparamagnetic iron oxide nanoparticles/polypeptides colloidal polyion complexes with high density of magnetic material

Bin Wang (1) , Olivier Sandre (2, 3) , Kunzhou Wang , Haishan Shi (4) , Kun Xiong (1) , Yu-Bin Huang (5) , Tingting Wu (6) , Minhao Yan (1) , Jérémie Courtois (1)

1 SWUST - Southwest University of Science and Technology [Mianyang]
2 LCPO - Laboratoire de Chimie des Polymères Organiques
3 Team 3 LCPO : Polymer Self-Assembly & Life Sciences
4 College of Chemistry and Materials Science, Jinan University
5 Changchun Institute of Applied Chemistry
6 Jinan University [Guangzhou]

Hypothesis: superparamagnetic iron oxide nanoparticles (SPIONs) are extensively used as building block of colloidal nanocomposites for biomedical applications. Strategies employed to embed them in a biodegradable and biocompatible polymer matrix often fail to achieve a high density of loading which would greatly benefit to applications such as imaging and hyperthermia. In this study, poly(acrylic acid) coated SPION (γ-Fe2O3-PAA) are self-assembled with hydrolysable poly(serine ester) by electrostatic complexation, leading to perfectly defined spherical particles with ultra-high density of magnetic material and an ability to auto-degrade into individual SPION and biocompatible byproducts. Experiments: self-assembly and auto-degradation of γ-Fe2O3-PAA/poly(serine ester) and γ-Fe2O3-PAA/poly(serine ester)-b-PEG colloidal particles are studied by light scattering and microscopy. Colloidal stability in bio-fluids, hyperthermia under alternating magnetic field, cellular uptake, cytotoxicity and degradation of γ-Fe2O3-PAA/poly(serine ester)-b-PEG in living cells are investigated. Findings: a remarkably slow electrostatic complexation leads to dense superparamagnetic γ-Fe2O3-PAA/poly(serine ester)-b-PEG polyion complexes (PICs) with controlled sizes (150 – 500 nm) and times of degradation in aqueous solvents (700 – 5000 h). The material shows good sustainability during hyperthermia, is well taken up by MC3T3 cells and non-cytotoxic. TEM images reveal a mechanism of degradation by “peeling” and fragmentation. In cells, PICs are reduced into individual SPIONs within 72 h.

Domaines: Polymères; Matière Molle [cond-mat.soft]; Biomatériaux

Bin Wang, Olivier Sandre, Kunzhou Wang, Haishan Shi, Kun Xiong, et al.. Auto-degradable and biocompatible superparamagnetic iron oxide nanoparticles/polypeptides colloidal polyion complexes with high density of magnetic material. Materials Science and Engineering: C, 2019, 104, pp.109920. ⟨10.1016/j.msec.2019.109920⟩. ⟨hal-02167584⟩