Superparamagnetic nanohybrids with cross-linked polymers providing higher in vitro stability
Résumé
A method for facile synthesis of high stable cross-linked superparamagnetic nanohybrids using pre-coating of biocompatible polymer, hydroxyl polyvinyl alcohol (PVA-OH) was demonstrated as a simple, controllable, fast, reproducible, and upscalable method. To improve stability, morphology and functionalization of the particles, the PVA-OH was first coated onto superparamagnetic iron oxide nanoparticle (SPION) surfaces before a cross-linking condensation with silica precursor. The obtained magnetic nanohybrid with compact silica layer was monodispersed with uniform morphology with the size of 50.0 ± 3.7 nm. The particles showed colloidal stability region at pH 7.35-7.45 which is applicable in biomedical applications and long shelf life upon storage of over 9 months. In vitro study in aspect of cytotoxicity and stability of the particle reveled that these original nanohybrids are non-toxic and highly robust in endosomal/lysosomal condition for up to 42 days without dissolution of the particles. In order to demonstrate the efficient targeting of the magnetic nanohybrid for their further use as targeting detection for example in MRI, we prepared folate reactive cross-linked magnetic nanohybrid which showed an interesting affinity to folate receptor (FR)-positive cervix (HeLa) cells. Our work to develop the characteristics of non-toxic and stable cross-linked magnetic nanohybrids would be beneficial for the further development of safer and more efficient targeting MRI contrast agent for diagnosis of various cancers.
Domaines
Matériaux
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