Development of a flow microsensor for selective detection of nitric oxide in the presence of hydrogen peroxide

Raquel Oliveira (1) , Catherine Sella (2) , Christelle Souprayen (2) , Eric Aït-Yahiatène (2) , Cyrine Slim (1) , Sophie Griveau (1) , Laurent Thouin (2) , Fethi Bedioui (1)

1 UTCBS - UM 4 (UMR 8258 / U1022) - Unité de Technologies Chimiques et Biologiques pour la Santé
2 PASTEUR - Processus d'Activation Sélective par Transfert d'Energie Uni-électronique ou Radiatif (UMR 8640)

The detection of reactive oxygen and nitrogen species is of utmost importance in several pathological situations. Indeed, these reactive species are biomarkers of oxidative stress and their real-time monitoring is crucial to adapt medical treatments. We report here on the electrochemical detection of nitric oxide (NO•) in the presence of hydrogen peroxide (H2O2). The detection was performed by using different sensing microdevices involving either static solutions in wells or flowing solutions in microfluidic channels. Furthermore, an original strategy was proposed to further enhance the selectivity of NO• detection at Pt/poly(eugenol) modified platinum electrodes by designing a dual-electrode microfluidic device based on a pre-electrolysis of interfering species including H2O2 at an upstream electrode prior to NO• detection.

Mots clés: poly(eugenol); oxidative stress; nitric oxide; microchannel; electrochemical sensor

Domaines: Chimie analytique; Chimie théorique et/ou physique

Raquel Oliveira, Catherine Sella, Christelle Souprayen, Eric Aït-Yahiatène, Cyrine Slim, et al.. Development of a flow microsensor for selective detection of nitric oxide in the presence of hydrogen peroxide. Electrochimica Acta, 2018, 286, pp.365-373. ⟨10.1016/j.electacta.2018.07.158⟩. ⟨hal-02159767⟩