One complete and three draft genome sequences of four Brochothrix thermosphacta strains, CD 337, TAP 175, BSAS1 3 and EBP 3070

Nassima Illikoud (1) , Christophe C. Klopp (2) , Alain Roulet (3) , Olivier Bouchez (3) , Nathalie Marsaud (4) , Emmanuel Jaffres (1) , Monique Zagorec (1)

1 SECALIM - SECurité des ALIments et Microbiologie
2 MIAT INRA - Unité de Mathématiques et Informatique Appliquées de Toulouse
3 GeT-PlaGe - Génome et Transcriptome - Plateforme Génomique
4 LISBP - Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés

Brochothrix thermosphacta is one of the dominant bacterial species associated with spoilage of chilled meat and seafood products through the production of various metabolites responsible for off-odors. However, metabolic pathways leading to meat and seafood spoilage are not all well known. The production of spoiling molecules seems to depend both on strains and on food matrix. Several B. thermosphacta genome sequences have been reported, all issued from meat isolates. Here, we report four genome sequences, one complete and three as drafts. The four B. thermosphacta strains CD 337, TAP 175, BSAS1 3, and EBP 3070 were isolated from different ecological niches (seafood or meat products either spoiled or not and bovine slaughterhouse). These strains known as phenotypically and genetically different were selected to represent intraspecies diversity. CD 337 genome is 2,594,337 bp long, complete and circular, containing 2593 protein coding sequences and 28 RNA genes. TAP 175, BSAS1 3, and EBP 3070 genomes are arranged in 57, 83, and 71 contigs, containing 2515, 2668, and 2611 protein-coding sequences, respectively. These genomes were compared with two other B. thermosphacta complete genome sequences. The main genome content differences between strains are phages, plasmids, restriction/modification systems, and cell surface functions, suggesting a similar metabolic potential but a different niche adaptation capacity.

Mots clés: Cooked shrimp; Bovine slaughterhouse; smoked salmon; Listeriaceae; Brochothrix thermosphacta; Spoilage; Chicken meat

Domaines: Sciences du Vivant [q-bio]

Nassima Illikoud, Christophe C. Klopp, Alain Roulet, Olivier Bouchez, Nathalie Marsaud, et al.. One complete and three draft genome sequences of four Brochothrix thermosphacta strains, CD 337, TAP 175, BSAS1 3 and EBP 3070. Standards in Genomic Sciences, 2018, 13 (1), pp.1-12. ⟨10.1186/s40793-018-0333-z⟩. ⟨hal-02154348⟩