Identification and Characterization of a Halotolerant, Cold-Active Marine Endo-β-1,4-Glucanase by Using Functional Metagenomics of Seaweed-Associated Microbiota

Marjolaine Martin (1) , Sophie Biver (1) , Sébastien Steels (1) , Tristan Barbeyron (2) , Murielle Jam (2) , Daniel Portetelle (1) , Gurvan Michel (2) , Micheline Vandenbol (2)

1 Gembloux Agro-Bio Tech [Gembloux]
2 LBI2M - Laboratoire de Biologie Intégrative des Modèles Marins

A metagenomic library was constructed from microorganisms associated with the brown alga Ascophyllum nodosum. Functional screening of this library revealed 13 novel putative esterase loci and two glycoside hydrolase loci. Sequence and gene cluster analysis showed the wide diversity of the identified enzymes and gave an idea of the microbial populations present during the sample collection period. Lastly, an endo-beta-1,4-glucanase having less than 50% identity to sequences of known cellulases was purified and partially characterized, showing activity at low temperature and after prolonged incubation in concentrated salt solutions.

Domaines: Biochimie [q-bio.BM]; Microbiologie et Parasitologie

Marjolaine Martin, Sophie Biver, Sébastien Steels, Tristan Barbeyron, Murielle Jam, et al.. Identification and Characterization of a Halotolerant, Cold-Active Marine Endo-β-1,4-Glucanase by Using Functional Metagenomics of Seaweed-Associated Microbiota. Applied and Environmental Microbiology, 2014, 80 (16), pp.4958-4967. ⟨10.1128/AEM.01194-14⟩. ⟨hal-02138133⟩