Antifungal activity of a Saharan strain of Actinomadura sp. ACD1 against toxigenic fungi and other pathogenic microorganisms

Abdelhadi Lahoum (1) , Adel Aouiche (1) , Noureddine Bouras (1, 2) , Carol Verheecke (3) , Hans-Peter Klenk (4) , Nasserdine Sabaou (1) , Florence Mathieu (3)

1 ENS Kouba-Alger - École normale supérieure - Kouba-Alger
2 Université de Ghardaïa
3 LGC - Laboratoire de Génie Chimique
4 Newcastle University [Newcastle]

A new strain of actinobacteria, designated ACD1, was isolated from a Saharan soil sample in the Hoggar region (Algeria). Morphological study led to this strain being classified as a member of the Actinomadura genus. Phylogenetic analysis based on the 16S rRNA gene showed that the strain is closely related to Actinomadura sediminis DSM 45500T (98.5% sequence similarity). Furthermore, strain ACD1 presented a strong activity against mycotoxigenic and phytopathogenic fungi, including Aspergillus and Fusarium strains, and other pathogenic microorganisms. The kinetics of antimicrobial activity were investigated on ISP-2, Bennett and TSB media. Four solvents (n-hexane, dichloromethane, ethyl acetate and n-butanol) were used for the extraction of the produced antibiotic. The highest antimicrobial activity was obtained using the butanolic extract from the ISP-2 medium after seven days of fermentation culture. The active antibiotic was purified by reverse-phase HPLC using a C18 column. The UV-visible and mass spectra were determined. The minimum inhibitory concentrations (MIC) of this antibiotic were determined against pathogenic microorganisms.

Mots clés: Actinobacterium; Actinomadura; Pathogenic microorganisms; Antimicrobial activity

Domaines: Génie chimique; Génie des procédés

Abdelhadi Lahoum, Adel Aouiche, Noureddine Bouras, Carol Verheecke, Hans-Peter Klenk, et al.. Antifungal activity of a Saharan strain of Actinomadura sp. ACD1 against toxigenic fungi and other pathogenic microorganisms. Journal of Medical Mycology = Journal de Mycologie Médicale, 2016, 26 (3), pp.193-200. ⟨10.1016/j.mycmed.2016.02.017⟩. ⟨hal-02134854⟩