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Journal articles
Antifungal activity of a Saharan strain of Actinomadura sp. ACD1 against toxigenic fungi and other pathogenic microorganisms
Abstract : A new strain of actinobacteria, designated ACD1, was isolated from a Saharan soil sample in the Hoggar region (Algeria). Morphological study led to this strain being classified as a member of the Actinomadura genus. Phylogenetic analysis based on the 16S rRNA gene showed that the strain is closely related to Actinomadura sediminis DSM 45500T (98.5% sequence similarity). Furthermore, strain ACD1 presented a strong activity against mycotoxigenic and phytopathogenic fungi, including Aspergillus and Fusarium strains, and other pathogenic microorganisms. The kinetics of antimicrobial activity were investigated on ISP-2, Bennett and TSB media. Four solvents (n-hexane, dichloromethane, ethyl acetate and n-butanol) were used for the extraction of the produced antibiotic. The highest antimicrobial activity was obtained using the butanolic extract from the ISP-2 medium after seven days of fermentation culture. The active antibiotic was purified by reverse-phase HPLC using a C18 column. The UV-visible and mass spectra were determined. The minimum inhibitory concentrations (MIC) of this antibiotic were determined against pathogenic microorganisms.
https://hal.archives-ouvertes.fr/hal-02134854
Contributor : Open Archive Toulouse Archive Ouverte (oatao) <>
Submitted on : Monday, May 20, 2019 - 5:34:02 PM
Last modification on : Monday, February 17, 2020 - 5:20:06 PM
Contributor : Open Archive Toulouse Archive Ouverte (oatao) <>
Submitted on : Monday, May 20, 2019 - 5:34:02 PM
Last modification on : Monday, February 17, 2020 - 5:20:06 PM
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