High resolution mapping of E.coli transcription elongation complex in situ reveals protein interactions with the non-transcribed strand.

Abstract : We have used chemical probes and UV light to perform a high resolution mapping of an Escherichia coli transcription elongation complex that was arrested in vivo by a protein readblock at a position distal to the promoter. The in situ probing data provide a precise picture of a constrained ternary complex in which the front edge of the polymerase is located at <6 bp from the catalytic center. Furthermore, our analyses reveal protein contacts with the non-transcribed strand within the arrested ternary complex. Thus, these results contribute substantially to the emerging view of a flexible transcription elongation complex in which the non-transcribed strand is an important regulatory element.
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https://hal.archives-ouvertes.fr/hal-02127225
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Submitted on : Monday, May 13, 2019 - 12:04:49 PM
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  • HAL Id : hal-02127225, version 1
  • PUBMED : 8895583

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M Guérin, M. Leng, A. Rachid Rahmouni. High resolution mapping of E.coli transcription elongation complex in situ reveals protein interactions with the non-transcribed strand.. EMBO Journal, EMBO Press, 1996, 15 (19), pp.5397-5407. ⟨hal-02127225⟩

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