Combination of Traction Assays and Multiphoton Imaging to Quantify Skin Biomechanics

Abstract : An important issue in tissue biomechanics is to decipher the relationship between the mechanical behavior at macroscopic scale and the organization of the collagen fiber network at microscopic scale. Here, we present a protocol to combine traction assays with multiphoton microscopy in ex vivo murine skin. This multiscale approach provides simultaneously the stress/stretch response of a skin biopsy and the collagen reorganization in the dermis by use of second harmonic generation (SHG) signals and appropriate image processing.
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Submitted on : Tuesday, April 30, 2019 - 1:29:19 PM
Last modification on : Tuesday, May 7, 2019 - 1:28:05 AM

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Stéphane Bancelin, Barbara Lynch, Christelle Bonod-Bidaud, Petr Dokládal, Florence Ruggiero, et al.. Combination of Traction Assays and Multiphoton Imaging to Quantify Skin Biomechanics. Sagi, Irit, Afratis, Nikolaos A. Collagen : Methods and Protocols, 1944, Humana Press, pp.145-155, 2019, Methods in Molecular Biology, 978-1-4939-9095-5. ⟨10.1007/978-1-4939-9095-5_11⟩. ⟨https://www.springer.com/us/book/9781493990948⟩. ⟨hal-02115604⟩

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