Identification of Nucleolus-Associated Chromatin Domains Reveals a Role for the Nucleolus in 3D Organization of the A. thaliana Genome

Frederic Pontvianne (1) , Marie-Christine Carpentier (1) , Nathalie Durut (1) , Veronika Pavlištová (2) , Karin Jaške , Šárka Schořová , Hugues Parrinello (3, 4) , Marine Rohmer (3) , Craig Pikaard (5) , Miloslava Fojtová , Jiří Fajkus (6) , Julio Sáez-Vasquez (1)

1 LGDP - Laboratoire Génome et développement des plantes
2 CEITEC MU - Central European Institute of Technology [Brno]
3 IGF MGX - Institut de Génomique Fonctionnelle - Montpellier GenomiX
4 BCM - BioCampus
5 Indiana University [Bloomington]
6 Mendel Centre for Plant Genomics and Proteomics [Brno, Czech Republic]

The nucleolus is the site of rRNA gene transcription,rRNA processing, and ribosome biogenesis. How-ever, the nucleolus also plays additional roles in thecell. We isolated nucleoli using fluorescence-acti-vated cell sorting (FACS) and identified nucleolus-associated chromatin domains (NADs) by deep seq-uencing, comparing wild-type plants and null mutantsfor the nucleolar protein NUCLEOLIN 1 (NUC1). NADsare primarily genomic regions with heterochromaticsignatures and include transposable elements (TEs),sub-telomeric regions, and mostly inactive protein-coding genes. However, NADs also include activer RNA genes and the entire short arm of chromosome4 adjacent to them. Innuc1null mutants, which alterrRNA gene expression and overall nucleolar structure,NADs are altered, telomere association with thenucle-olus is decreased, and telomeres become shorter.Collectively, our studies reveal roles for NUC1 and the nucleolus in the spatial organization of chromosomes as well as telomere maintenance

Domaines: Sciences du Vivant [q-bio]

Frederic Pontvianne, Marie-Christine Carpentier, Nathalie Durut, Veronika Pavlištová, Karin Jaške, et al.. Identification of Nucleolus-Associated Chromatin Domains Reveals a Role for the Nucleolus in 3D Organization of the A. thaliana Genome. Cell Reports, 2016, 16 (6), pp.1574-1587. ⟨10.1016/j.celrep.2016.07.016⟩. ⟨hal-02115369⟩