Formation of acetyl-Ser-Asp-Lys-Pro, a new regulator of the hematopoietic system, through enzymatic processing of thymosin beta 4.

M Lenfant (1) , Catherine Grillon (1) , J. Rieger (2) , D Sotty , J. Wdzieczak-Bakala (1)

1 ICSN - Institut de Chimie des Substances Naturelles
2 inconnu

The demonstration that AcSDKP, a new regulator of the hematopoietic system, is formed in the bone marrow by a one-step enzymatic maturation processing of thymosin beta 4 (T beta 4) is presented. AcSDKP and T beta 4 were both detected in bone marrow cells (BMC). Incubation of [3H]T beta 4 with either intact or lysed BMC led to the formation of [3H]AcSDKP, whereas the labeled tetrapeptide was not degraded under these conditions. Model enzymatic degradation of T beta 4 carried out with bacterial enzymes suggests that a mammalian endoproteinase Asp-N might be involved in the formation of AcSDKP through the specific cleavage of the Pro4-Asp5 peptidic bond of T beta 4. In contrast, alpha-prolyl-endopeptidase was ineffective in carrying out a similar processing.

Domaines: Sciences du Vivant [q-bio]

M Lenfant, Catherine Grillon, J. Rieger, D Sotty, J. Wdzieczak-Bakala. Formation of acetyl-Ser-Asp-Lys-Pro, a new regulator of the hematopoietic system, through enzymatic processing of thymosin beta 4.. Annals of the New York Academy of Sciences, 1991, 628, pp.115-25. ⟨hal-02114977⟩