Skip to Main content Skip to Navigation
Journal articles

Shigella Phagocytic Vacuolar Membrane Remnants Participate in the Cellular Response to Pathogen Invasion and Are Regulated by Autophagy

Abstract : Intracellular pathogens like Shigella flexneri enter host cells by phagocytosis. Once inside, the pathogen breaks the vacuolar membrane for cytosolic access. The fate and function of the vacuolar membrane remnants are not clear. Examining Shigella-infected nonmyeloid cells, we observed that proteins associated with vacuolar membrane remnants are polyubiquinated, recruit the autophagy marker LC3 and adaptor p62, and are targeted to autophagic degradation. Further, inflammasome components and caspase-1 were localized to these membranes and correlated with dampened inflammatory response and necrotic cell death. In Atg4B mutant cells in which autophagosome maturation is blocked, polyubiquitinated proteins and P62 accumulated on membrane remnants, and as in autophagy-deficient Atg5(-/-) cells, the early inflammatory and cytokine response was exacerbated. Our results suggest that host membranes, after rupture by an invading cytoplasm-targeted bacterium, contribute to the cellular responses to infection by acting as a signaling node, with autophagy playing a central role in regulating these responses.
Complete list of metadata

https://hal.archives-ouvertes.fr/hal-02109149
Contributor : Sandra LACAS-GERVAIS Connect in order to contact the contributor
Submitted on : Wednesday, April 24, 2019 - 4:09:39 PM
Last modification on : Tuesday, February 22, 2022 - 1:04:04 PM

Links full text

Identifiers

Collections

Citation

Nicolas Dupont, Sandra Lacas-Gervais, Julie Bertout, Irit Paz, Barbara Freche, et al.. Shigella Phagocytic Vacuolar Membrane Remnants Participate in the Cellular Response to Pathogen Invasion and Are Regulated by Autophagy. Cell Host and Microbe, Elsevier, 2009, 6 (2), pp.137-149. ⟨10.1016/j.chom.2009.07.005⟩. ⟨hal-02109149⟩

Share

Metrics

Record views

30