Molecular diagnosis of drug-resistant Ttuberculosis : a literature review

Thi Ngoc Anh Nguyen (1) , Véronique Le Berre (2) , Anne-Laure Bañuls (3) , Thi van Anh Nguyen (1)

1 NIHE - Institut National d'Hygiène et d'Épidémiologie de Hanoi
2 LISBP - Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés
3 MIVEGEC-GeneSys - Du gène à l'écosystème

Drug-resistant tuberculosis is a global health problem that hinders the progress of tuberculosis eradication programs. Accurate and early detection of drug-resistant tuberculosis is essential for effective patient care, for preventing tuberculosis spread, and for limiting the development of drug-resistant strains. Culture-based drug susceptibility tests are the gold standard method for the detection of drug-resistant tuberculosis, but they are time-consuming and technically challenging, especially in low- and middle-income countries. Nowadays, different nucleic acid-based assays that detect gene mutations associated with resistance to drugs used to treat tuberculosis are available. These tests vary in type and number of targets and in sensitivity and specificity. In this review, we will describe the available molecular tests for drug-resistant tuberculosis detection and discuss their advantages and limitations.

Mots clés: diagnosis; mutations; diagnosis; molecular markers; challenges; tuberculosis; drug resistance

Domaines: Sciences du Vivant [q-bio]; Ingénierie biomédicale; Microbiologie et Parasitologie

Thi Ngoc Anh Nguyen, Véronique Le Berre, Anne-Laure Bañuls, Thi van Anh Nguyen. Molecular diagnosis of drug-resistant Ttuberculosis : a literature review. Frontiers in Microbiology, 2019, 10, pp.1-12. ⟨10.3389/fmicb.2019.00794⟩. ⟨hal-02106689⟩