Probing dynamic processes in the eye at multiple spatial and temporal scales with multimodal full field OCT

Jules Scholler (1) , Viacheslav Mazlin (1) , Olivier Thouvenin (1) , Kassandra Groux (1) , Peng Xiao (2) , José-Alain Sahel (3, 4, 5) , Mathias Fink (1) , Claude Boccara (1) , Kate Grieve (3, 4)

1 IL - Institut Langevin - Ondes et Images (UMR7587)
2 SYSU - Sun Yat-sen University [Guangzhou]
3 Institut de la Vision
4 CHNO - Centre Hospitalier National d'Ophtalmologie des Quinze-Vingts
5 University of Pittsburgh School of Medicine

We describe recent technological progress in multimodal en face full-field optical coherence tomography that has allowed detection of slow and fast dynamic processes in the eye. We show that by combining static, dynamic and fluorescence contrasts we can achieve label-free high-resolution imaging of the retina and anterior eye with temporal resolution from milliseconds to several hours, allowing us to probe biological activity at subcellular scales inside 3D bulk tissue. Our setups combine high lateral resolution over a large field of view with acquisition at several hundreds of frames per second which make it a promising tool for clinical applications and biomedical studies. Its contactless and non-destructive nature is shown to be effective for both following in vitro sample evolution over long periods of time and for imaging of the human eye in vivo.

Domaines: Imagerie; Organes des sens

Jules Scholler, Viacheslav Mazlin, Olivier Thouvenin, Kassandra Groux, Peng Xiao, et al.. Probing dynamic processes in the eye at multiple spatial and temporal scales with multimodal full field OCT. Biomedical optics express, 2019, 10 (2), pp.731. ⟨10.1364/boe.10.000731⟩. ⟨hal-02024973⟩