Sensing Proteins through Nanopores: Fundamental to Applications
Résumé
Proteins subjected to an electric field and forced to pass through a nanopore induce blockades of ionic current that depend on the protein and nanopore characteristics and interactions between them. Recent advances in the analysis of these blockades have highlighted a variety of phenomena that can be used to study protein translocation and protein folding, to probe single-molecule catalytic reactions in order to obtain kinetic and thermodynamic information, and to detect protein–antibody complexes, proteins with DNA and RNA aptamers, and protein–pore interactions. Nanopore design is now well controlled, allowing the development of future biotechnologies and medicine applications.