Helium Plasma Jet for Cell Membrane Permeabilization and Drug Delivery Application

Abstract : Drug delivery in cancer cells combining low cytotoxicity and high efficiency remains to be optimized with physical techniques. Several studies have shown that cold atmospheric plasmas (CAPs) have demonstrated their relevance in biomedical applications and are prone to be quite useful tools in medicine. Plasma could be used to deliver chemotherapy [1] or nucleic acids [2] via enhancement of cell permeabilization. Parameters needed for helium plasma to induce cell membrane permeabilization on cancer cells were investigated (Fig.1). A frequency of 100 Hz for 100 s, corresponding to 10,000 pulses, with an applied voltage of 14 kV was used. Both plasma electric field and endocytosis involvement in molecular uptake were studied [3]. Kinetics of plasma-induced permeabilization were also studied. Highest permeabilization efficiency was observed when propidium iodide was added after treatment (up to 40% of propidium iodide positive cells in both HeLa and 4T1). No significant toxicity was observed in these conditions as the viability in HeLa cells was measured to be of 80%. A treatment combining doxorubicin and plasma also showed improvement of cell membrane permeabilization. Figure 1: Round-shaped permeabilization spot of propidium iodide in 4T1 cells after plasma jet treatment References [1] W. Zhu, S.-J. Lee, N. J. Castro, D. Yan, M. Keidar, and L. G. Zhang, Scientific Report, 6, (2016). [2] M. Jinno, Y. Ikeda, H. Motomura, Y. Kido and S. Satoh. Archives Biochemistry Biophysics, 605, 59-66 (2016) [3] V. Vijayarangan, A. Delalande, S. Dozias, J.-M. Pouvesle, C. Pichon, and E. Robert, IEEE Transactions on Radiation and Plasma Medical Sciences, 2, 1-7 (2017).
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Contributor : Jean-Michel Pouvesle <>
Submitted on : Wednesday, December 5, 2018 - 5:36:52 PM
Last modification on : Tuesday, May 21, 2019 - 5:26:28 PM

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Vinodini Vijayarangan, Anthony Delalande, Sébastien Dozias, Jean-Michel Pouvesle, Chantal Pichon, et al.. Helium Plasma Jet for Cell Membrane Permeabilization and Drug Delivery Application. SFNano 2018, SFN, Dec 2018, Montpellier, France. ⟨hal-01946070⟩

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