Over-elongation of centrioles in cancer promotes centriole amplification and chromosome missegregation

Abstract : Centrosomes are the major microtubule organising centres of animal cells. Deregulation in their number occurs in cancer and was shown to trigger tumorigenesis in mice. However, the incidence, consequence and origins of this abnormality are poorly understood. Here, we screened the NCI-60 panel of human cancer cell lines to systematically analyse centriole number and structure. Our screen shows that centriole amplification is widespread in cancer cell lines and highly prevalent in aggressive breast carcinomas. Moreover, we identify another recurrent feature of cancer cells: centriole size deregulation. Further experiments demonstrate that severe centriole over-elongation can promote amplification through both centriole fragmentation and ectopic procentriole formation. Furthermore, we show that overly long centrioles form over-active centrosomes that nucleate more microtubules, a known cause of invasiveness, and perturb chromosome segregation. Our screen establishes centriole amplification and size deregulation as recurrent features of cancer cells and identifies novel causes and consequences of those abnormalities.
Type de document :
Article dans une revue
Nature Communications, Nature Publishing Group, 2018, 9 (1), pp.1258. 〈10.1038/s41467-018-03641-x〉
Liste complète des métadonnées

https://hal.archives-ouvertes.fr/hal-01939139
Contributeur : Mouniati Colo <>
Soumis le : jeudi 29 novembre 2018 - 11:19:34
Dernière modification le : vendredi 30 novembre 2018 - 01:22:17

Lien texte intégral

Identifiants

Citation

Gaëlle Marteil, Adan Guerrero, André Vieira, Bernardo De Almeida, Pedro Machado, et al.. Over-elongation of centrioles in cancer promotes centriole amplification and chromosome missegregation. Nature Communications, Nature Publishing Group, 2018, 9 (1), pp.1258. 〈10.1038/s41467-018-03641-x〉. 〈hal-01939139〉

Partager

Métriques

Consultations de la notice

27