Multiple Insecticide Resistances in the Disease Vector Culex p. Quinquefasciatus from Western Indian Ocean

Nicolas Pocquet (1) , Pascal Milesi (2) , Patrick Makoundou (2) , Sandra Unal (2) , Betty Zumbo (3) , Célestine Atyame Nten (2) , Frédéric Darriet (4) , Jean-Sébastien Dehecq (5) , Julien Thiria (6) , Ambicadutt Bheecarry , Diana Iyaloo , Mylène Weill (2) , Fabrice Chandre (4) , Pierrick Labbe (2)

1 MIVEGEC - Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle
2 UMR ISEM - Institut des Sciences de l'Evolution de Montpellier
3 Service de lutte anti-vectorielle
4 MIVEGEC-VCG - Vector Control Group
5 Agence Régionale de Santé Océan Indien
6 Direction Régionale des Affaires Sanitaires et Sociales de La Réunion

Several mosquito-borne diseases affect the Western Indian Ocean islands. Culex pipiens quinquefasciatus is one of these vectors and transmits filariasis, Rift Valley and West Nile viruses and the Japanese encephalitis. To limit the impact of these diseases on public health, considerable vector control efforts have been implemented since the 50s, mainly through the use of neurotoxic insecticides belonging to Organochlorines (OC), Organophosphates (OP) and pyrethroids (PYR) families. However, mosquito control failures have been reported on site, and they were probably due to the selection of resistant individuals in response to insecticide exposure. In this study, we used different approaches to establish a first regional assessment of the levels and mechanisms of resistance to various insecticides. Bioassays were used to evaluate resistance to various insecticides, enzyme activity was measured to assess the presence of metabolic resistances through elevated detoxification, and molecular identification of known resistance alleles was investigated to determine the frequency of target-site mutations. These complementary approaches showed that resistance to the most used insecticides families (OC, OP and PYR) is widespread at a regional scale. However, the distribution of the different resistance genes is quite heterogeneous among the islands, some being found at high frequencies everywhere, others being frequent in some islands and absent in others. Moreover, two resistance alleles displayed clinal distributions in Mayotte and La Réunion, probably as a result of a heterogeneous selection due to local treatment practices. These widespread and diverse resistance mechanisms reduce the capacity of resistance management through classical strategies (e.g. insecticide rotation). In case of a disease outbreak, it could undermine the efforts of the vector control services, as only few compounds could be used. It thus becomes urgent to find alternatives to control populations of Cx. p. quinquefasciatus in the Indian Ocean.

Domaines: Evolution [q-bio.PE]; Génétique des populations [q-bio.PE]; Génétique animale

Nicolas Pocquet, Pascal Milesi, Patrick Makoundou, Sandra Unal, Betty Zumbo, et al.. Multiple Insecticide Resistances in the Disease Vector Culex p. Quinquefasciatus from Western Indian Ocean. PLoS ONE, 2013, 8 (10), pp.e77855. ⟨10.1371/journal.pone.0077855⟩. ⟨hal-01938096⟩