D-dimer Quantification from Autologous Red Blood Cells Agglutination by a Lens-free Imaging Device

M. Huet (1) , M. Cubizolles (1) , P. Pouteau , V. Poher , A. Buhot (2)

1 CEA-LETI - Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information
2 CREAB - Chimie pour la Reconnaissance et l’Etude d’Assemblages Biologiques

Quantification of biomarkers closest to the patient remains an impressive challenge for the development of personalized medicine. The detection of D-dimer, a fibrin degradation product, indicative of deep vein thrombosis, pulmonary embolism or stroke is a particular biomarker of interest. In this study, we developed a quantitative assay based on the measurement of autologous red blood cells agglutination by a lens-free device. An indicator, obtained from the shape of the gray level histogram of the images, allows us for a linear quantification of D-dimer opening the door for a Point-of-Care device.

Mots clés: lens-free detection; Red blood cells agglutination; D-dimer quantification

Domaines: Biotechnologies

M. Huet, M. Cubizolles, P. Pouteau, V. Poher, A. Buhot. D-dimer Quantification from Autologous Red Blood Cells Agglutination by a Lens-free Imaging Device. Procedia Technology, 2017, 27, pp.167 - 168. ⟨10.1016/j.protcy.2017.04.073⟩. ⟨hal-01925307⟩