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Journal articles
The Polycomb group protein EZH2 directly controls DNA methylation
Emmanuelle Viré
Carmen Brenner
Rachel Deplus
1
Loïc Blanchon
2
Mario Fraga
3
Céline Didelot
Lluis Morey
Aleyde van Eynde
David Bernard
4
Jean-Marie Vanderwinden
5
Mathieu Bollen
6
Manel Esteller
7
Luciano Di Croce
Yvan de Launoit
8
François Fuks
1
Emmanuelle Viré
Author
Carmen Brenner
Author
Rachel Deplus 1
Author
Loïc Blanchon 2
Author IdHAL : loic-blanchon
David Bernard 4
Author
Jean-Marie Vanderwinden 5
Author
Mathieu Bollen 6
Author
Manel Esteller 7
Author
1
ULB - Université libre de Bruxelles (Avenue Franklin Roosevelt 50 - 1050 Bruxelles - Belgium)
2
GReD - Génétique, Reproduction et Développement (Facultés de Médecine et de Pharmacie, TSA 50400, 28 Place Henri Dunant, 63001 Clermont-Ferrand - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U931 (101, rue de Tolbiac, 75013 Paris - France)
- UCA [2017-2020] - Université Clermont Auvergne [2017-2020] : UMR6293 (49, bd François-Mitterrand / CS 60032 / 63001 Clermont-Ferrand Cedex 1 - France)
- CNRS - Centre National de la Recherche Scientifique : UMR6293 (France)
4
Orange Labs - Cesson-Sévigné (Orange) (France)
5
Laboratory of Neurophysiology [Brussels] (808 route de Lennik
1070 Brussels - Belgium)
- ULB - Université libre de Bruxelles (Avenue Franklin Roosevelt 50 - 1050 Bruxelles - Belgium)
6
LUT - Luleå University of Technology (SE-97187 Luleå, Sweden - Sweden)
8
Institut de Biologie de Lille (Universités de Lille 1 et 2 IFR 142 59021 Lille - France)
- Institut Pasteur de Lille (1 Rue du Professeur Calmette - Lille Cedex - 59019 - France)
- RIIP - Réseau International des Instituts Pasteur (25, rue du Dr Roux 75724 Paris Cedex 15 - France)
- CNRS - Centre National de la Recherche Scientifique : UMR8161 (France)
Abstract : The establishment and maintenance of epigenetic gene silencing is fundamental to cell determination and function. The essential epigenetic systems involved in heritable repression of gene activity are the Polycomb group (PcG) proteins and the DNA methylation systems. Here we show that the corresponding silencing pathways are mechanistically linked. We find that the PcG protein EZH2 (Enhancer of Zeste homolog 2) interacts-within the context of the Polycomb repressive complexes 2 and 3 (PRC2/3)-with DNA methyltransferases (DNMTs) and associates with DNMT activity in vivo. Chromatin immunoprecipitations indicate that binding of DNMTs to several EZH2-repressed genes depends on the presence of EZH2. Furthermore, we show by bisulphite genomic sequencing that EZH2 is required for DNA methylation of EZH2-target promoters. Our results suggest that EZH2 serves as a recruitment platform for DNA methyltransferases, thus highlighting a previously unrecognized direct connection between two key epigenetic repression systems.
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Journal articles
Complete list of metadatas
https://hal.uca.fr/hal-01918637
Contributor : Loic Blanchon <>
Submitted on : Sunday, November 11, 2018 - 9:45:29 PM
Last modification on : Tuesday, February 23, 2021 - 11:34:04 AM
Contributor : Loic Blanchon <>
Submitted on : Sunday, November 11, 2018 - 9:45:29 PM
Last modification on : Tuesday, February 23, 2021 - 11:34:04 AM
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Identifiers
- HAL Id : hal-01918637, version 1
- DOI : 10.1038/nature04431
- PUBMED : 16357870
Collections
PRES_CLERMONT | CNRS | RIIP | RIIP_LILLE | GRED
Citation
Emmanuelle Viré, Carmen Brenner, Rachel Deplus, Loïc Blanchon, Mario Fraga, et al.. The Polycomb group protein EZH2 directly controls DNA methylation. Nature, Nature Publishing Group, 2006, 439 (7078), pp.871 - 874. ⟨10.1038/nature04431⟩. ⟨hal-01918637⟩
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