Impact of the chemicals, essential for the purification process of strict Fe-hydrogenase, on the corrosion of mild steel

Abstract : The influence of additional chemical molecules, necessary for the purification process of [Fe]-hydrogenase from Clostridium acetobutylicum, was studied on the anaerobic corrosion of mild steel. At the end of the purification process, the pure [Fe-Fe]-hydrogenase was recovered in a Tris-HCl medium containing three other chemicals at low concentration: DTT, dithionite and desthiobiotin. Firstly, mild steel coupons were exposed in parallel to a 0.1 M pH7 Tris-HCl medium with or without pure hydrogenase. The results showed that hydrogenase and the additional molecules were in competition, and the electrochemical response could not be attributed solely to hydrogenase. Then, solutions with additional chemicals of different compositions were studied electrochemically. DTT polluted the electrochemical signal by increasing the Eoc by 35 mV 24 h after the injection of 300 μL of control solutions with DTT, whereas it drastically decreased the corrosion rate by increasing the charge transfer resistance (Rct 10 times the initial value). Thus, DTT was shown to have a strong antagonistic effect on corrosion and was removed from the purification process. An optimal composition of the medium was selected (0.5 mM dithionite, 7.5 mM desthiobiotin) that simultaneously allowed a high activity of hydrogenase and a lower impact on the electrochemical response for corrosion tests.
Complete list of metadatas

Cited literature [51 references]  Display  Hide  Download

https://hal.archives-ouvertes.fr/hal-01886386
Contributor : Archive Ouverte Prodinra <>
Submitted on : Monday, November 5, 2018 - 8:17:48 AM
Last modification on : Thursday, November 21, 2019 - 9:59:34 AM
Long-term archiving on: Wednesday, February 6, 2019 - 1:51:16 PM

File

Rouvre_20537.pdf
Files produced by the author(s)

Identifiers

Citation

Ingrid Rouvre, Charles Gauquelin, Isabelle Meynial Salles, Regine Basseguy. Impact of the chemicals, essential for the purification process of strict Fe-hydrogenase, on the corrosion of mild steel. Bioelectrochemistry, Elsevier, 2016, 109, pp.9-23. ⟨10.1016/j.bioelechem.2015.12.006⟩. ⟨hal-01886386⟩

Share

Metrics

Record views

51

Files downloads

91