Quantitative FLIM-FRET Microscopy to Monitor Nanoscale Chromatin Compaction In Vivo Reveals Structural Roles of Condensin Complexes

David Llères (1) , Aymeric P Bailly (2) , Aurélien Perrin (2) , David G Norman (3) , Dimitris P Xirodimas (2) , Robert Feil (1)

1 IGMM - Institut de Génétique Moléculaire de Montpellier
2 CRBM - Centre de recherche en Biologie cellulaire de Montpellier
3 University of Dundee

Highlights d Meiotic chromosomes in living C. elegans display heterogeneous nanoscale compaction d Heterochromatin has a compacted nanoscale organization controlled by HP1 and SETDB1 homologs d Tandem repeat-enriched ectopic chromosomes acquire heterochromatic structure in meiotic cells d Condensin I and II play essential roles in meiotic heterochromatin compaction

Domaines: Sciences du Vivant [q-bio]; Biochimie, Biologie Moléculaire; Biologie cellulaire; Biologie du développement; Cancer

David Llères, Aymeric P Bailly, Aurélien Perrin, David G Norman, Dimitris P Xirodimas, et al.. Quantitative FLIM-FRET Microscopy to Monitor Nanoscale Chromatin Compaction In Vivo Reveals Structural Roles of Condensin Complexes. Cell Reports, 2017, 18 (7), pp.1791 - 1803. ⟨10.1016/j.celrep.2017.01.043⟩. ⟨hal-03027061⟩