Type 1 regulatory T cells specific for collagen type II as an efficient cell-based therapy in arthritis
Résumé
Introduction: Regulatory T (Treg) cells play a crucial role in preventing autoimmune diseases and are an ideal
target for the development of therapies designed to suppress inflammation in an antigen-specific manner.
Type 1 regulatory T (Tr1) cells are defined by their capacity to produce high levels of interleukin 10 (IL-10), which
contributes to their ability to suppress pathological immune responses in several settings. The aim of this study was
to evaluate the therapeutic potential of collagen type II–specific Tr1 (Col-Treg) cells in two models of rheumatoid
arthritis (RA) in mice.
Methods: Col-Treg clones were isolated and expanded from collagen-specific TCR transgenic mice. Their cytokine
secretion profile and phenotype characterization were studied. The therapeutic potential of Col-Treg cells was
evaluated after adoptive transfer in collagen-antibody– and collagen-induced arthritis models. The in vivo
suppressive mechanism of Col-Treg clones on effector T-cell proliferation was also investigated.
Results: Col-Treg clones are characterized by their specific cytokine profile (IL-10highIL-4negIFN-γint) and mediate
contact-independent immune suppression. They also share with natural Tregs high expression of GITR, CD39 and
granzyme B. A single infusion of Col-Treg cells reduced the incidence and clinical symptoms of arthritis in both
preventive and curative settings, with a significant impact on collagen type II antibodies. Importantly, injection of
antigen-specific Tr1 cells decreased the proliferation of antigen-specific effector T cells in vivo significantly.
Conclusions: Our results demonstrate the therapeutic potential of Col-Treg cells in two models of RA, providing
evidence that Col-Treg could be an efficient cell-based therapy for RA patients whose disease is refractory to current
treatments.
Domaines
Immunologie
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