Characterization of the Virulome of Staphylococcus Aureus by a Highly Multiplex Approach with Scout-MRM

Abstract : The emergence of antibiotic-resistant bacteria strains is seriously threatening human life, as recently underlined by the World Health Organization. The case of Staphylococcus aureus, S.a. is of particular concern, whose forms resisting to vancomycin or methicillin caused numerous patients death in Hospitals. It is also crucial to better understand how mutations of the bacterial genome induce the emergence of new resistant strains. The sensitivity and multiplexing possibilities of mass spectrometric (MS) approaches appears particularly relevant to tackle such problems. We thus developed a targeted assay covering 90 key proteins involved in S.a. virulence and antibiotic resistance using a novel and a highly multiplexed targeted-MS approach, Scout-MRM. The virulome assay has been implemented for deciphering the proteogenomic links across a clinical collection of 230 S.a. strains.
Type de document :
Communication dans un congrès
Clinical Mass Spectrometry MSACL 2018 EU, Sep 2018, Salzburg, Austria
Domaine :
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https://hal.archives-ouvertes.fr/hal-01866344
Contributeur : Agnès Bussy <>
Soumis le : lundi 3 septembre 2018 - 11:55:53
Dernière modification le : mercredi 6 février 2019 - 10:07:53

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  • HAL Id : hal-01866344, version 1

Citation

Nicolas Mouton, Marianne Pivard, Romain Carrière, Sophie Ayciriex, Florence Couzon, et al.. Characterization of the Virulome of Staphylococcus Aureus by a Highly Multiplex Approach with Scout-MRM. Clinical Mass Spectrometry MSACL 2018 EU, Sep 2018, Salzburg, Austria. 〈hal-01866344〉

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