Needle in a haystack: involvement of the copepod Paracartia grani in the life-cycle of the oyster pathogen Marteilia refringens

Abstract : Marteilia refringens is a major pathogen of the European flat oyster, Ostrea edulis Linnaeus. Since its description, the life-cycle of this protozoan parasite has eluded discovery. Attempts to infect oysters experimentally have been unsuccessful and led to the hypothesis of a complex life-cycle involving several hosts. Knowledge of this life-cycle is of central importance in order to manage oyster disease. However, the exploration of M. refringens life-cycle has been previously limited by the detection tools available and the tremendous number of species to be screened in enzootic areas. In this study, these two restrictions were circumvented by the use of both molecular detection tools and a mesocosm with low biodiversity. Screening of the entire fauna of the pond for M. refringens DNA was systematically undertaken using PCR. Here, we show that the copepod Paracartia(Acartia) grani is a host of M. refringens. Not only was DNA of M. refringens consistently detected in P. grani but also the presence of the parasite in the ovarian tissues was demonstrated using in situ hybridization. Finally, successful experimental transmissions provided evidence that P. grani can be infected from infected flat oysters.
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https://hal.archives-ouvertes.fr/hal-01854092
Contributeur : Pierre-Guy Sauriau <>
Soumis le : lundi 6 août 2018 - 14:46:43
Dernière modification le : jeudi 16 août 2018 - 16:30:02

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Corine Audemard, Frédérique Le Roux, Antoine Barnaud, C.M. Collins, Benoit Sautour, et al.. Needle in a haystack: involvement of the copepod Paracartia grani in the life-cycle of the oyster pathogen Marteilia refringens. Parasitology, Cambridge University Press (CUP), 2002, 124 (3), pp.315-323. 〈10.1017/S0031182001001111〉. 〈hal-01854092〉

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