Fast and high resolution single-cell BRET imaging

Elise Goyet (1) , Nathalie Bouquier (1) , Vincent Ollendorff (2) , Julie Perroy (1)

1 IGF - Institut de Génomique Fonctionnelle
2 DMEM - Dynamique Musculaire et Métabolisme

Resonance Energy Transfer (RET)-based technologies are used to report protein-protein interactions in living cells. Among them, Bioluminescence-initiated RET (BRET) provides excellent sensitivity but the low light intensity intrinsic to the bioluminescent process hampers its use for the localization of protein complexes at the sub-cellular level. Herein we have characterized the methodological conditions required to reliably perform single-cell BRET imaging using an extremely bright luciferase, Nanoluciferase (Nluc). With this, we achieved an unprecedented performance in the field of protein-protein interaction imaging in terms of temporal and spatial resolution, duration of signal stability, signal sensitivity and dynamic range. As proof-of-principle, an Nluc-containing BRET-based sensor of ERK activity enabled the detection of subtle, transient and localized variations in ERK activity in neuronal dendritic spines, induced by the activation of endogenous synaptic NMDA receptors. This development will improve our comprehension of both the spatio-temporal dynamics of protein-protein interactions and the activation patterns of specific signaling pathways.

Mots clés: bioluminescence

Mots clés: énergie de résonance

Domaines: Biotechnologies; Physiologie [q-bio.TO]

Elise Goyet, Nathalie Bouquier, Vincent Ollendorff, Julie Perroy. Fast and high resolution single-cell BRET imaging. Scientific Reports, 2016, 6, 12 p. ⟨10.1038/srep28231⟩. ⟨hal-01837646⟩