Stromal cells control the epithelial residence of DCs and memory T cells by regulated activation of TGF-beta

J. Mohammed , L.K. Beura , A. Bobr , B. Astry , B. Chicoine , S.W. Kashem , N.E. Welty , B.Z. Igyarto , S. Wijeyesinghe , E.A. Thompson , C. Matte , L. Bartholin (1) , A. Kaplan , D. Sheppard , A.G. Bridges , W.D. Shlomchik , D. Masopust , D.H. Kaplan

1 UNICANCER/CRCL - Centre de Recherche en Cancérologie de Lyon

Cells of the immune system that reside in barrier epithelia provide a first line of defense against pathogens. Langerhans cells (LCs) and CD8+ tissue-resident memory T cells (TRM cells) require active transforming growth factor-beta1 (TGF-beta) for epidermal residence. Here we found that integrins alphavbeta6 and alphavbeta8 were expressed in non-overlapping patterns by keratinocytes (KCs) and maintained the epidermal residence of LCs and TRM cells by activating latent TGF-beta. Similarly, the residence of dendritic cells and TRM cells in the small intestine epithelium also required alphavbeta6. Treatment of the skin with ultraviolet irradiation decreased integrin expression on KCs and reduced the availability of active TGF-beta, which resulted in LC migration. Our data demonstrated that regulated activation of TGF-beta by stromal cells was able to directly control epithelial residence of cells of the immune system through a novel mechanism of intercellular communication

Mots clés: France; Medicine; Cells; Dermatology; Dendritic Cells; microbiology; immunology; Laboratories; pathology; Growth; Skin; Immune System; Epithelium; Integrins; Stromal Cells; Transforming Growth Factor beta1

Domaines: Cancer

J. Mohammed, L.K. Beura, A. Bobr, B. Astry, B. Chicoine, et al.. Stromal cells control the epithelial residence of DCs and memory T cells by regulated activation of TGF-beta. Nature Immunology, 2016, 17, pp.414--421. ⟨10.1038/ni.3396⟩. ⟨hal-01812677⟩