Skip to Main content Skip to Navigation
Journal articles

Size and Content of the Sex-Determining Region of the Y Chromosome in Dioecious Mercurialis annua, a Plant with Homomorphic Sex Chromosomes

Abstract : Dioecious plants vary in whether their sex chromosomes are heteromorphic or homomorphic, but even homomorphic sex chromosomes may show divergence between homologues in the non-recombining, sex-determining region (SDR). Very little is known about the SDR of these species, which might represent particularly early stages of sex-chromosome evolution. Here, we assess the size and content of the SDR of the diploid dioecious herb Mercurialis annua, a species with homomorphic sex chromosomes and mild Y-chromosome degeneration. We used RNA sequencing (RNAseq) to identify new Y-linked markers for M. annua. Twelve of 24 transcripts showing male-specific expression in a previous experiment could be amplified by polymerase chain reaction (PCR) only from males, and are thus likely to be Y-linked. Analysis of genome-capture data from multiple populations of M. annua pointed to an additional six male-limited (and thus Y-linked) sequences. We used these markers to identify and sequence 17 sex-linked bacterial artificial chromosomes (BACs), which form 11 groups of non-overlapping sequences, covering a total sequence length of about 1.5 Mb. Content analysis of this region suggests that it is enriched for repeats, has low gene density, and contains few candidate sex-determining genes. The BACs map to a subset of the sex-linked region of the genetic map, which we estimate to be at least 14.5 Mb. This is substantially larger than estimates for other dioecious plants with homomorphic sex chromosomes, both in absolute terms and relative to their genome sizes. Our data provide a rare, high-resolution view of the homomorphic Y chromosome of a dioecious plant.
Complete list of metadatas

Cited literature [91 references]  Display  Hide  Download

https://hal.archives-ouvertes.fr/hal-01806943
Contributor : Anne-Gestion Labo Duputié <>
Submitted on : Tuesday, May 26, 2020 - 12:24:09 PM
Last modification on : Saturday, May 30, 2020 - 1:48:02 PM

File

2018_Velstos_Genes_1.pdf
fileSource_public_editor

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Collections

Citation

Paris Veltsos, Guillaume Cossard, Emmanuel Beaudoing, Genséric Beydon, Dessislava Savova Bianchi, et al.. Size and Content of the Sex-Determining Region of the Y Chromosome in Dioecious Mercurialis annua, a Plant with Homomorphic Sex Chromosomes. Genes, MDPI, 2018, 9 (6), pp.277. ⟨10.3390/genes9060277⟩. ⟨hal-01806943⟩

Share

Metrics

Record views

121