A conductometric creatinine biosensor prepared through contact printing of polyvinyl alcohol/polyethyleneimine based enzymatic membrane

Abstract : A conductometric creatinine biosensor was prepared through creatinine deaminase entrapped into a polyvinyl alcohol/polyethyleneimine/gold nanoparticles composite film, previously deposited on the surface of a PDMS stamp and then, after glutaraldehyde coupling, transferred on interdigitated microelectrodes by contact printing method. The detection of creatinine in a sucrose background solution, revealed a wide linear calibration plot (10–600 μM creatinine concentration range), with a limit of detection of 2 μM (S/N = 3), a good repeatability. The resulting creatinine biosensor exhibits a drastic increase of detection range compared to that of the biosensor prepared by simple dropping: 10–300 μM creatinine concentration range. The developed biosensor was validated by determining creatinine in spiked artificial blood serum.
Document type :
Journal articles
Liste complète des métadonnées

https://hal.archives-ouvertes.fr/hal-01767656
Contributor : Agnès Bussy <>
Submitted on : Monday, April 16, 2018 - 2:08:18 PM
Last modification on : Saturday, July 21, 2018 - 1:14:42 AM

Identifiers

Collections

Citation

Mohamed Braiek, Mohamed Amine Djebbi, François Chateaux, Anne Bonhommé, Roberto Vargiolu, et al.. A conductometric creatinine biosensor prepared through contact printing of polyvinyl alcohol/polyethyleneimine based enzymatic membrane. Microelectronic Engineering, Elsevier, 2018, 187-188, pp.43 - 49. ⟨10.1016/j.mee.2017.11.018⟩. ⟨hal-01767656⟩

Share

Metrics

Record views

103