A conductometric creatinine biosensor prepared through contact printing of polyvinyl alcohol/polyethyleneimine based enzymatic membrane

Abstract : A conductometric creatinine biosensor was prepared through creatinine deaminase entrapped into a polyvinyl alcohol/polyethyleneimine/gold nanoparticles composite film, previously deposited on the surface of a PDMS stamp and then, after glutaraldehyde coupling, transferred on interdigitated microelectrodes by contact printing method. The detection of creatinine in a sucrose background solution, revealed a wide linear calibration plot (10–600 μM creatinine concentration range), with a limit of detection of 2 μM (S/N = 3), a good repeatability. The resulting creatinine biosensor exhibits a drastic increase of detection range compared to that of the biosensor prepared by simple dropping: 10–300 μM creatinine concentration range. The developed biosensor was validated by determining creatinine in spiked artificial blood serum.
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Microelectronic Engineering, Elsevier, 2018, 187-188, pp.43 - 49. 〈10.1016/j.mee.2017.11.018〉
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https://hal.archives-ouvertes.fr/hal-01767656
Contributeur : Agnès Bussy <>
Soumis le : lundi 16 avril 2018 - 14:08:18
Dernière modification le : samedi 21 juillet 2018 - 01:14:42

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Mohamed Braiek, Mohamed Amine Djebbi, François Chateaux, Anne Bonhommé, Roberto Vargiolu, et al.. A conductometric creatinine biosensor prepared through contact printing of polyvinyl alcohol/polyethyleneimine based enzymatic membrane. Microelectronic Engineering, Elsevier, 2018, 187-188, pp.43 - 49. 〈10.1016/j.mee.2017.11.018〉. 〈hal-01767656〉

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