SENSITIVE DETECTION OF H2B ANTIBODIES BY ENHANCED ELISA USING POROUS SILICON MICROCAVITIES

Sathish Kumar Ramakrishan , Marta Martin-Fernandez (1) , Thierry Cloitre (1) , Vivechana Agarwal , F.J.G. Cuisinier (2) , Csilla Gergely (1)

1 L2C - Laboratoire Charles Coulomb
2 LBN - Laboratoire de Bioingénierie et NanoSciences

We are interested in the early stage diagnosis of H2B, a clinically important autoantigen in various severe illnesses (can-cers, systemic lupus, rheumatoid arthritis). Accordingly, we have developed a porous-silicon (PSi) based enzyme-linked immunosorbent assay (ELISA) for sensitive detection of H2B antibodies, where H2B antigen were bound to a Si binding 12mer peptide [1] that act as an efficient linker to the surface. The proposed method is a simple, inexpensive sensing meth-od employing a PSi photonic substrate, a material affinity peptide, and a smart phone CCD detector to achieve a 100-fold (~ 10 fg/ml) increase in sensitivity compared to the standard ELISA (0.01 -0.1 ng/ml).

Domaines: Biophysique [physics.bio-ph]

Sathish Kumar Ramakrishan, Marta Martin-Fernandez, Thierry Cloitre, Vivechana Agarwal, F.J.G. Cuisinier, et al.. SENSITIVE DETECTION OF H2B ANTIBODIES BY ENHANCED ELISA USING POROUS SILICON MICROCAVITIES . Porous Semiconductors - Science and Technology 2018, Mar 2018, La Grande Motte, Montpellier, France. ⟨hal-01745847⟩