High throughput single cell counting in droplet-based microfluidics

Heng Lu (1) , Ouriel Caen (1) , Jérémy Vrignon (2) , Eleonora Zonta (1) , Zakaria El Harrak (1) , Philippe Nizard (1) , Jean-Christophe Baret (2) , Valéry Taly (1)

1 MEPPOT - U1147 - Médecine Personnalisée, Pharmacogénomique, Optimisation Thérapeutique
2 CRPP - Centre de Recherche Paul Pascal

Droplet-based microfluidics is extensively and increasingly used for high-throughput single-cell studies. However, the accuracy of the cell counting method directly impacts the robustness of such studies. We describe here a simple and precise method to accurately count a large number of adherent and non-adherent human cells as well as bacteria. Our microfluidic hemocytometer provides statistically relevant data on large populations of cells at a high-throughput, used to characterize cell encapsulation and cell viability during incubation in droplets.

Domaines: Matière Condensée [cond-mat]

Heng Lu, Ouriel Caen, Jérémy Vrignon, Eleonora Zonta, Zakaria El Harrak, et al.. High throughput single cell counting in droplet-based microfluidics. Scientific Reports, 2017, 7 (1), ⟨10.1038/s41598-017-01454-4⟩. ⟨hal-01687572⟩