Polyproline as a Minimal Antifreeze Protein Mimic That Enhances the Cryopreservation of Cell Monolayers

Ben Graham (1) , Trisha L. Bailey (1) , Joseph R. J. Healey (1) , Moreno Marcellini (2) , Sylvain Deville (2) , Matthew I. Gibson (1)

1 University of Warwick [Coventry]
2 LSFC - Laboratoire de Synthèse et Fonctionnalisation des Céramiques

Tissue engineering,g ene therapy, drug screening, and emerging regenerative medicine therapies are fundamentally reliant on high-quality adherent cell culture,b ut current methods to cryopreserve cells in this format can give lowc ell yields and require large volumes of solvent " antifreezes ". Herein, we report polyproline as am inimum (bio)synthetic mimic of antifreezep roteins that is accessible by solution, solid-phase,a nd recombinant methods.W ed emonstrate that polyproline has ice recrystallisation inhibition activity linked to its amphipathic helix and that it enhances the DMSO cryopreservation of adherent cell lines.P olyproline mayb e av ersatile additive in the emerging field of macromolecular cryoprotectants.

Domaines: Matériaux

Ben Graham, Trisha L. Bailey, Joseph R. J. Healey, Moreno Marcellini, Sylvain Deville, et al.. Polyproline as a Minimal Antifreeze Protein Mimic That Enhances the Cryopreservation of Cell Monolayers. Angewandte Chemie, 2017, 129 (50), pp.16157 - 16160. ⟨10.1002/ange.201706703⟩. ⟨hal-01685648⟩