Skip to Main content Skip to Navigation
Journal articles

Overexpression of the class D MADS-box gene Sl-AGL11 impacts fleshy tissue differentiation and structure in tomato fruits

Abstract : MADS-box transcription factors are key elements of the genetic networks controlling flower and fruit development. Among these, the class D clade gathers AGAMOUS-like genes which are involved in seed, ovule, and funiculus development. The tomato genome comprises two class D genes, Sl-AGL11 and Sl-MBP3 , both displaying high expression levels in seeds and in central tissues of young fruits. The potential effects of Sl-AGL11 on fruit development were addressed through RNAi silencing and ectopic expression strategies. Sl-AGL11-down-regulated tomato lines failed to show obvious phenotypes except a slight reduction in seed size. In contrast, Sl-AGL11 overexpression triggered dramatic modifications of flower and fruit structure that include: the conversion of sepals into fleshy organs undergoing ethylene-dependent ripening, a placenta hypertrophy to the detriment of locular space, starch and sugar accumulation, and an extreme softening that occurs well before the onset of ripening. RNA-Seq transcriptomic profiling high-lighted substantial metabolic reprogramming occurring in sepals and fruits, with major impacts on cell wall-related genes. While several Sl-AGL11-related phenotypes are reminiscent of class C MADS-box genes (TAG1 and TAGL1), the modifications observed on the placenta and cell wall and the Sl-AGL11 expression pattern suggest an action of this class D MADS-box factor on early fleshy fruit development.
Complete list of metadata

Cited literature [70 references]  Display  Hide  Download

https://hal.archives-ouvertes.fr/hal-01643490
Contributor : Open Archive Toulouse Archive Ouverte (oatao) <>
Submitted on : Tuesday, November 21, 2017 - 3:17:10 PM
Last modification on : Saturday, June 12, 2021 - 3:01:06 AM

File

Huang_18643.pdf
Files produced by the author(s)

Identifiers

Collections

Citation

Baowen Huang, Jean-Pierre-Amans Routaboul, Mingchun Liu, Wei Deng, Elie Maza, et al.. Overexpression of the class D MADS-box gene Sl-AGL11 impacts fleshy tissue differentiation and structure in tomato fruits. Journal of Experimental Botany, Oxford University Press (OUP), 2017, 68 (17), pp.4869-4884. ⟨10.1093/jxb/erx303⟩. ⟨hal-01643490⟩

Share

Metrics

Record views

171

Files downloads

550