Planar Optical Nano-antennas Resolve Cholesterol-Dependent Nanoscale Heterogeneities in the Plasma Membrane of Living Cells

Abstract : Optical nano-antennas can efficiently confine light into nanoscopic hotspots enabling single-molecule detection sensitivity at biological relevant conditions. This innovative approach to breach the diffraction limit offers a versatile platform to investigate the dynamics of individual biomolecules in living cell membranes and their partitioning into cholesterol-dependent lipid nanodomains. Here, we present optical nano-antenna arrays with accessible surface hotspots to study the characteristic diffusion dynamics of phosphoethanolamine (PE) and sphingomyelin (SM) in the plasma membrane of living cells at the nanoscale. Fluorescence burst analysis
Complete list of metadatas

Cited literature [18 references]  Display  Hide  Download

https://hal.archives-ouvertes.fr/hal-01621274
Contributor : Jérome Wenger <>
Submitted on : Thursday, April 12, 2018 - 7:12:59 PM
Last modification on : Thursday, March 7, 2019 - 4:14:10 PM

File

1711.06617.pdf
Files produced by the author(s)

Identifiers

Collections

Citation

Raju Regmi, Pamina M. Winkler, Valentin Flauraud, Kyra J. E. Borgman, Carlo Manzo, et al.. Planar Optical Nano-antennas Resolve Cholesterol-Dependent Nanoscale Heterogeneities in the Plasma Membrane of Living Cells. Nano Letters, American Chemical Society, 2017, 17 (10), pp.6295 - 6302. ⟨10.1021/acs.nanolett.7b02973⟩. ⟨hal-01621274⟩

Share

Metrics

Record views

152

Files downloads

79