A Combination of Histological, Physiological, and Proteomic Approaches Shed Light on Seed Desiccation Tolerance of the Basal Angiosperm Amborella trichopoda.

Matthieu Villegente (1) , Philippe Marmey (2) , Claudette C. Job (3, 4) , Marc M. Galland (5) , Gwendal Cueff (5) , Beatrice Godin (5) , Loïc Rajjou (5) , Thierry Balliau (6) , Michel Zivy (6) , Bruno Fogliani (7, 1) , Valérie Burtet-Sarramegna (8) , Dominique D. Job (3, 4)

1 UNC - Université de la Nouvelle-Calédonie
2 UMR DIADE - Diversité, adaptation, développement des plantes
3 MAP - Microbiologie, adaptation et pathogénie
4 BayerCropScience
5 IJPB - Institut Jean-Pierre Bourgin
6 GQE-Le Moulon - Génétique Quantitative et Evolution - Le Moulon (Génétique Végétale)
7 IAC - Institut Agronomique Néo-Calédonien
8 ISEA - Institut de sciences exactes et appliquées

Desiccation tolerance allows plant seeds to remain viable in a dry state for years and even centuries. To reveal potential evolutionary processes of this trait, we have conducted a shotgun proteomic analysis of isolated embryo and endosperm from mature seeds of Amborella trichopoda, an understory shrub endemic to New Caledonia that is considered to be the basal extant angiosperm. The present analysis led to the characterization of 415 and 69 proteins from the isolated embryo and endosperm tissues, respectively. The role of these proteins is discussed in terms of protein evolution and physiological properties of the rudimentary, underdeveloped, Amborella embryos, notably considering that the acquisition of desiccation tolerance corresponds to the final developmental stage of mature seeds possessing large embryos.

Mots clés: Amborella trichopoda; Basal angiosperms; Seeds; Desiccation tolerance

Mots clés: Proteomics

Domaines: Amélioration des plantes; Biotechnologies; Bio-Informatique, Biologie Systémique [q-bio.QM]; Organisation et fonctions cellulaires [q-bio.SC]; Biochimie [q-bio.BM]; Biologie moléculaire; Génomique, Transcriptomique et Protéomique [q-bio.GN]; Réseaux moléculaires [q-bio.MN]; Alimentation et Nutrition

Matthieu Villegente, Philippe Marmey, Claudette C. Job, Marc M. Galland, Gwendal Cueff, et al.. A Combination of Histological, Physiological, and Proteomic Approaches Shed Light on Seed Desiccation Tolerance of the Basal Angiosperm Amborella trichopoda.. Proteomes, 2017, 5 (3), pp.19. ⟨10.3390/proteomes5030019⟩. ⟨hal-01615957⟩