Towards a standardization of biomethane potential tests

Christof Holliger 1, * Madalena Alves 2 Diana Andrade 3 Irini Angelidaki 4 Sergi Astals 5 Urs Baier 6 Claire Bougrier 7 Pierre Buffière 8 Marta Carballa 9 Vinnie de Wilde 10 Florian Ebertseder 3 Belén Fernández 11 Elena Ficara 12 Ioannis Fotidis 4 Jean-Claude Frigon 13 Hélène Fruteau de Laclos 14 Dara S. M. Ghasimi 15 Gabrielle Hack 1 Mathias Hartel 3 Joern Heerenklage 16 Ilona Sarvari Horvath 17 Pavel Jenicek 18 Konrad Koch 19 Judith Krautwald 6 Javier Lizasoain 20 Jing Liu 21 Lona Mosberger 6 Mihaela Nistor 21 Hans Oechsner 22 João Vítor Oliveira 23 Mark Paterson 24 André Pauss 25 Sébastien Pommier 26 Isabella Porqueddu 12 Francisco Raposo 27 Thierry Ribeiro 28 Florian Rüsch Pfund 6 Sten Strömberg 21 Michel Torrijos 29 Miriam Van Eekert 30 Jules Van Lier 15 Harald Wedwitschka 31 Isabella Wierinck 32
* Corresponding author
Abstract : Production of biogas from different organic materials is a most interesting source of renewable energy. The biomethane potential (BMP) of these materials has to be determined to get insight in design parameters for anaerobic digesters. Although several norms and guidelines for BMP tests exist, inter-laboratory tests regularly still show high variability of BMPs for the same substrate. A workshop was held in June 2015, in Leysin, Switzerland, with over 40 attendees from 30 laboratories around the world, to agree on common solutions to the conundrum of inconsistent BMP test results. This paper presents the consensus of the intense roundtable discussions and cross-comparison of methodologies used in respective laboratories. Compulsory elements for the validation of BMP results were defined. They include the minimal number of replicates, the request to carry out blank and positive control assays, a criterion for the test duration, details on BMP calculation, and last but not least criteria for rejection of the BMP tests. Finally, recommendations on items that strongly influence the outcome of BMP tests such as inoculum characteristics, substrate preparation, test setup, and data analysis are presented to increase the probability of obtaining validated and reproducible results.
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Christof Holliger, Madalena Alves, Diana Andrade, Irini Angelidaki, Sergi Astals, et al.. Towards a standardization of biomethane potential tests. Water Science and Technology, IWA Publishing, 2016, 74 (11), pp.2515-2522. ⟨10.2166/wst.2016.336⟩. ⟨hal-01606690⟩

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