Assessment of genetic diversity among cultivated Pearl millet (Pennisetum glaucum, Poaceae) accessions from Benin, West Africa

Kifouli Adeoti (1) , Gustave Djedatin (2) , Ebenezer Ewedje (2) , Thierry Beulé (3, 4, 5) , Sylvain Santoni (6) , Alain Rival (7) , Estelle Jaligot (3, 4, 5)

1 Département de Biologie Végétale, Faculté des Sciences et Techniques, LAMITA
2 Faculté des Sciences et Techniques de Dassa Zoumè
3 UMR DIADE - Diversité, adaptation, développement des plantes
4 IRD [France-Ouest] - Institut de Recherche pour le Développement
5 UM - Université de Montpellier
6 UMR AGAP - Amélioration génétique et adaptation des plantes méditerranéennes et tropicales
7 Cirad-Dgdrs - Direction Générale Déléguée à la Recherche et à la Stratégie

Simple sequence repeat (SSR) molecular markers were used for genetic diversity analysis and population structure of the cultivated Pearl millet in Benin, West Africa. In order to assess the level of genetic diversity, 14 polymorphic SSR markers were used to screen 114 accessions from different agro-ecological zones in Benin. SSR markers were found to reveal a total of 57 alleles with an average of 4.071 allele per locus. Genetic diversity index varied from 0.099 to 0.633 with an average of 0.405. The average observed heterozygosity was found to reach 0.425. The analysis of molecular variance showed no real differentiation between regions. Only 5% of genetic variation was observed between samples collected from north-eastern and north-western region. A high level of variation (95%) was observed among accessions. Moreover, both principal component analysis (PCA) and the dendrogram obtained from the genetic distance among accessions revealed the absence of any specific structuration of accessions from each region under study. Our results confirmed diversity among cultivated Pearl millet in Benin and such diversity is not clustering according to geographical patterns.

Mots clés: cereal; simple sequence repeat (SSR) markers; genetic variability; genêtic variation; pearl millet (plant)

Mots clés: diversité génétique; pennisetum glaucum; poaceae; benin; afrique de l'ouest

Domaines: Biologie végétale; Sciences agricoles

Kifouli Adeoti, Gustave Djedatin, Ebenezer Ewedje, Thierry Beulé, Sylvain Santoni, et al.. Assessment of genetic diversity among cultivated Pearl millet (Pennisetum glaucum, Poaceae) accessions from Benin, West Africa. African Journal of Biotechnology, 2017, 16 (15), pp.782-790. ⟨10.5897/ajb2017.15898⟩. ⟨hal-01602666⟩