Development of 18 microsatellite markers in Rhododendron ferrugineum (Ericaceae) for investigating genetic structure at margins

Olivia Charrier (1, 2, 3) , Chloé E. L. Delmas (1, 2, 3) , André Pornon (1, 2, 3) , Nathalie Escaravage (1, 2, 3)

1 EDB - Evolution et Diversité Biologique
2 ENFA - Ecole Nationale de Formation Agronomique de Toulouse
3 CNRS - Centre National de la Recherche Scientifique

Eighteen novel microsatellite markers were developed in Rhododendron ferrugineum using pyrosequencing technology. They were tested on populations in the Alps, Jura and Pyrenees to study genetic diversity at the species range margins. Protection of populations at margins is important for conserving the genetic diversity of species. We found 2–7 alleles per locus. The expected and observed heterozygosity ranged from 0.07 to 0.79 and from 0.05 to 0.85, respectively. These loci were successfully amplified in 22 other Rhododendron species and four Ericaceae species. These markers will be useful for investigating the genetic structure of R. ferrugineum and related species at their distribution margins.

Mots clés: Ericaceae; microsatellite markers; population genetics; pyrosequencing; microsatellite repeats; azalea

Mots clés: pyrénées; alpes; jura; pyroséquençage; marqueur microsatellite; rhododendron

Domaines: Sciences du Vivant [q-bio]

Olivia Charrier, Chloé E. L. Delmas, André Pornon, Nathalie Escaravage. Development of 18 microsatellite markers in Rhododendron ferrugineum (Ericaceae) for investigating genetic structure at margins. Conservation Genetics Resources, 2013, 5 (2), pp.473-477. ⟨10.1007/s12686-012-9831-2⟩. ⟨hal-01601838⟩