Structure of T-DNA in plants regenerated from roots transformed by Agrobacterium rhizogenes strain A4
Résumé
the structure of the t-dna in ri-transformedplants of brassica napus, nicotiana plumbaginifolia and nicotianatabacum was analysed. all the plants studied presenta particular phenotype with wrinkled leaves. the t-dnais composed of two parts: tl and tr. the size of thetl-dna (19-20 kb) seems to be almost constant, exceptin n. tabacum where it is shorter. the tr-dna can beabsent, and its size varies from about 5-28 kb, with twopredominant lengths. the smaller size does not include theregion homologous to the tms genes of the pti t-dna.the copy number varies from one to four copies per plantgenome. tl and tr-dna are not always present in thesame copy number, but in some cases are linked together