Monitoring Biological Cell Flow within a Mimicking Capillary Device with Impedance Measurement

Tieying Xu, (1) , Maria Lizarralde, (2) , Wassim El Nemer (2) , Bruno Le Pioufle (1) , Olivier Français (3)

1 SATIE-SIAME - Systèmes d'Information et d'Analyse Multi-Echelles
2 BIGR (UMR_S_1134 / U1134) - Biologie Intégrée du Globule Rouge
3 ESYCOM - Electronique, Systèmes de communication et Microsystèmes

This paper presents a microfluidic system, characterizing the red blood cell (RBC) deformability through its transit time in a microchannel, which dimensions reproducing blood microcirculation capillary. Electrodes are integrated within the microchannel for electrical measurements. The transit time is measured with the electrical blockade produced when the RBC squeezes in the microchannel (d = 5 μm diameter, while the cell diameter is 8 μm). In order todetermine most suitable electrical frequency range for the voltage applied to excitation electrodes, FEM numerical simulation of the bioimpedance is achieved and presented in this paper. We investigated the frequency response of the bioimpedance taking into account the electrode polarization, the dielectric properties of the medium, a single shell model for RBC and finally the RBC position within the microchannel.

Mots clés: microfluidic; bioimpedance; sickle cell disease; mimetic chip

Domaines: Micro et nanotechnologies/Microélectronique

Tieying Xu,, Maria Lizarralde,, Wassim El Nemer, Bruno Le Pioufle, Olivier Français. Monitoring Biological Cell Flow within a Mimicking Capillary Device with Impedance Measurement. Eurosensors 2017, Sep 2017, Paris, France. pp.3 - 6, ⟨10.3390/proceedings1040517⟩. ⟨hal-01592142⟩