Coding in 2D: Using intentional dispersity to enhance the information capacity of sequence-coded polymer barcodes
Résumé
A 2D approach was studied for the design of polymer-based molecular barcodes. Uniform oligo(alkoxyamine amide)s, containing a monomer-coded binary message, were synthesized by orthogonal solid-phase chemistry. In particular, sets of oligomers with different chain-lengths were prepared. The physical mixture of these uniform oligomers leads to an intentional dispersity (1 st dimension fingerprint), which can be measured by electrospray mass spectrometry. Furthermore, the monomer sequence of each component of the mass distribution can be analyzed by tandem mass spectrometry (2 nd dimension sequencing). By summing the sequence information of all components, a binary message can be read. As a proof of concept, a 4-bytes extended ASCII-coded message was written on a set of six uniform oligomers. This message was read from the smallest to largest oligomer in the first dimension and from the α-to ω-terminus in the second dimension. Alternatively, a 3-bytes sequence was written on a set of five oligomers, which was read in the opposite direction, i.e. from the tallest to smallest oligomer in the first dimension and from ω-to α-terminus in the second dimension. In both cases, the coded binary information was recovered.
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