Dicationic Lipophosphoramidates as DNA Carriers

Mathieu Mével (1) , Tristan Montier (2) , François Lamarche (1) , Pascal Delépine (2) , Tony Le Gall (2) , Jean-Jacques Yaouanc (1) , Paul-Alain Jaffres (1) , Dominique Cartier (1) , Pierre Lehn (2) , Jean-Claude Clément (1)

1 CEMCA - Chimie, Electrochimie Moléculaires et Chimie Analytique
2 INSERM U613, IFR148

Lipophosphoramidates with two different permanent cations as polar heads were synthesized and evaluated for their gene transfer activity. Physicochemical measurements (particle size, zeta potentials) and gel retardation assays were also performed. In vitro biological evaluation was conducted with A542 and HeLa cell lines, and cytotoxicity determined by a chemiluminescent assay. The set of results indicates that, on the whole, dicationic lipophosphoramidates constitute an interesting alternative to their monocationic analogues.

Domaines: Chimie

Mathieu Mével, Tristan Montier, François Lamarche, Pascal Delépine, Tony Le Gall, et al.. Dicationic Lipophosphoramidates as DNA Carriers. Bioconjugate Chemistry, 2007, 18 (5), pp.1604 - 1611. ⟨10.1021/bc070089z⟩. ⟨hal-01581379⟩