Structure Change from Bet -Strand and Turn to alpha-Helix in Histone H2A-H2B Induced by DNA Damage Response

Y Izumi (1) , F Fujii (1) , F Wien (2) , C Houée-Lévin (3) , S Lacombe (4) , D Salado-Leza (4) , E Porcel (4) , R Masoud (3) , S Yamamoto (5) , Matthieu M. Refregiers (2) , M-A. Hervé Du Penhoat (6) , A Yokoya (1)

1 Quantum Beam Science Center
2 SSOLEIL - Synchrotron SOLEIL
3 LCPO - Laboratoire de Chimie Physique D'Orsay
4 ISMO - Institut des Sciences Moléculaires d'Orsay
5 Ibaraki University
6 IMPMC - Institut de minéralogie, de physique des matériaux et de cosmochimie

Using synchrotron radiation-based circular dichroism spectroscopy, we found that the DNA damage response induces an increase of α-helix structure and a decrease of β-strand and turn structures in histone H2A-H2B extracted from x-irradiated human HeLa cells. The structural alterations correspond to the assumption that an average of eight amino acid residues form new α-helix structures at 310 K. We propose the structural transition from β-strand and turn structures to an α-helix structure in H2A-H2B as a novel, to our knowledge, process involved in the DNA damage response.

Mots clés: Synchrotron radiation; circular dichroism; chromatin remodeling; post-translational modification; DNA repair; histone dynamics

Domaines: Biophysique; Radiochimie

Y Izumi, F Fujii, F Wien, C Houée-Lévin, S Lacombe, et al.. Structure Change from Bet -Strand and Turn to alpha-Helix in Histone H2A-H2B Induced by DNA Damage Response. Biophysical Journal, 2016, 111 (1), pp.69-78. ⟨10.1016/j.bpj.2016.06.002⟩. ⟨hal-01570956⟩