Study of the polymorphism of metallothioneins by ion spray mass spectrometry.

Étude du polymorphisme des métallothionéines par spectrométrie de masse à source Ion-spray

H. Chassaigne (1) , Ryszard Lobinski (1, 2)

1 LCABIE - Laboratoire de Chimie Analytique Bio-Inorganique et Environnement
2 IPREM - Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux

Ion-spray mass spectrometry (IS-MS) is shown to be an attractive technique to identify and to differentiate among the sub-isoforms of metallothioneins (MT). Different modes of application of IS-MS are discussed using the example of MT-2 rabbit liver prepurified by size-exclusion and anion-exchange chromatog. Direct IS-MS mode, microbore HPLC with online IS-MS detection of fully cadmiated (Cd7-MT isoforms), HPLC with post-column acidification and detection of the apo-MT isoforms by IS-MS, and with post-column acidification and detection of the Cd ion by IS-MS are discussed.

Mots clés: polymorphism metallothionein ion spray mass spectrometry

Domaines: Chimie analytique

H. Chassaigne, Ryszard Lobinski. Étude du polymorphisme des métallothionéines par spectrométrie de masse à source Ion-spray. Analusis, 1998, 26, pp.M65-M67. ⟨10.1051/analusis:199826060065⟩. ⟨hal-01559798⟩