Use of Synchrotron-Based Techniques to Elucidate Metal Uptake and Metabolism in Plants
Résumé
Synchrotron techniques have become key components of the toolbox for studying the mechanisms involved in metal(loid) uptake and metabolism in plants. Most widely used techniques in this field include micro-X-ray fluorescence (μXRF) for imaging the distribution of elements in plant tissues and cells and quantifying them, and X-ray absorption spectroscopy (XAS) for determining their chemical forms. Recent advances in terms of spatial resolution, sensitivity and versatility of the sample environment have opened new perspectives for the study of trace elements at the micro- and nanoscale with a minimal perturbation of the sample. Sample conditioning remains a key issue for the study of metals in plants. Cryogenic sample environments allow work on hydrated systems, with a limited risk of metal remobilization and changes in speciation. Still, radiation damage should be monitored carefully, especially for high-flux spectrometers. In addition, progress in software for data analysis has facilitated data mining and integration of results from various techniques. This chapter presents the principle and the basics of data analysis for μXRF imaging and tomography, XAS and micro-Fourier transform infrared spectromicroscopy (μFTIR). Major results obtained on Ni, Cd, Zn, Se, As, Cu, Mn and nanoparticles in hyperaccumulating and nonaccumulating plants are presented. Complementary approaches including histochemical techniques, micro and nanoscopic techniques using electron- or ion beams, and laser ablation coupled with inductively coupled plasma mass spectrometry (ICP-MS) are also presented, and key results reviewed. Finally, there is also great interest in coupling synchrotron techniques, which is possible on more and more beamlines, and also in coupling synchrotron techniques with other approaches such as the ones mentioned above; perspectives in this area are discussed. © 2013 Elsevier Inc.