Boron-doped Diamond Electrodes Modified with Fe 3 O 4 @Au Magnetic Nanocomposites as Sensitive Platform for Detection of a Cancer Biomarker, Interleukin-8.

Mohamed Braiek 1 Yi Yang 2 Carole Farre 1 Carole Chaix 1 François Bessueille 3 Abdoullatif Baraket 4 Abdelhamid Errachid 4 Aidong Zhang 2 Nicole Jaffrezic-Renault 1, *
* Auteur correspondant
1 Interfaces & biosensors - Interfaces & biocapteurs
ISA - Institut des Sciences Analytiques
3 Surfaces
ISA - Institut des Sciences Analytiques
4 Micro & Nanobiotechnologies
ISA - Institut des Sciences Analytiques
Abstract : Interleukin-8 (IL-8), a dimeric protein composed of two identical subunits, is overexpressed in different human cancers, among them pancreatic cancers and head and neck cancers. There is a strong demand for monitoring of this biomarker protein for early cancer detection. A new concept of label-free impedimetric immunosensors for IL-8 detection is described in this work. A nanocomposite of gold nanoparticles (AuNPs) decorating a magnetic Fe3O4 core is synthesized using cysteamine as linker, and characterized using TEM and UV-Vis spectrometry. After immobilization of these Fe3O4@Au magnetic nanocomposites on a boron doped diamond (BDD) electrode using a simple magnet, anti-human IL-8 monoclonal antibody is linked to the AuNPs, previously functionalized with thioctic acid. The functionalization of the immunosensor was characterized using two electrochemical techniques: cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS), performed in phosphate buffer with ferro/ferricyanide as the redox probe. The EIS technique was used for affinity assays: antibody-Antigen binding. A linear relationship between the increment in the electron transfer resistance (R-CT) and the logarithmic value of IL-8 concentration was observed between 0.1pg/mL and 1000pg/mL. The limit of detection (LOD) was estimated at 0.03pg/mL.
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Electroanalysis, Wiley-VCH Verlag, 2016, 28 (8), pp.1810-1816. 〈10.1002/elan.201600060〉
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Soumis le : samedi 24 juin 2017 - 18:07:42
Dernière modification le : jeudi 8 février 2018 - 11:10:30

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Mohamed Braiek, Yi Yang, Carole Farre, Carole Chaix, François Bessueille, et al.. Boron-doped Diamond Electrodes Modified with Fe 3 O 4 @Au Magnetic Nanocomposites as Sensitive Platform for Detection of a Cancer Biomarker, Interleukin-8.. Electroanalysis, Wiley-VCH Verlag, 2016, 28 (8), pp.1810-1816. 〈10.1002/elan.201600060〉. 〈hal-01546541〉

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