Scout-MRM: Multiplexed Targeted Mass Spectrometry-Based Assay without Retention Time Scheduling Exemplified by Dickeya dadantii Proteomic Analysis during Plant Infection

Abstract : Targeted mass spectrometry of a surrogate peptide panel is a powerful method to study the dynamics of protein networks, but chromatographic time scheduling remains a major limitation for dissemination and implementation of robust and large multiplexed assays. We unveil a Multiple Reaction Monitoring method (Scout-MRM) where the use of spiked scout peptides triggers complex transition lists, regardless of the retention time of targeted surrogate peptides. The interest of Scout-MRM method regarding the retention time independency, multiplexing capability, reproducibility, and putative interest in facilitating method transfer was illustrated by a 782-peptide-plex relative assay targeting 445 proteins of the phytopathogen Dickeya dadantii during plant infection.
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https://hal.archives-ouvertes.fr/hal-01518912
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Submitted on : Friday, May 5, 2017 - 3:31:43 PM
Last modification on : Thursday, February 21, 2019 - 10:34:03 AM

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Blandine Rougemont, Sebastien Gallo, Sophie Ayciriex, Romain Carrière, Hubert Hondermarck, et al.. Scout-MRM: Multiplexed Targeted Mass Spectrometry-Based Assay without Retention Time Scheduling Exemplified by Dickeya dadantii Proteomic Analysis during Plant Infection. Analytical Chemistry, American Chemical Society, 2017, 89 (3), pp.1421-1426 ⟨10.1021/acs.analchem.6b03201⟩. ⟨hal-01518912⟩

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