A bacterial two-hybrid genome fragment library for deciphering regulatory networks of the opportunistic pathogen Pseudomonas aeruginosa.

Laetitia Houot (1) , Adeline Fanni , Sophie De Bentzmann (1) , Christophe Bordi (1)

1 LISM - Laboratoire d'ingénierie des systèmes macromoléculaires

Bacterial gene regulation is controlled by complex regulatory cascades which integrate input environmental signals and adapt specific and adequate output cellular responses. These complex networks are far from being elucidated, in particular in Pseudomonas aeruginosa. In the present study, we developed bacterial two-hybrid genome fragment libraries of the P. aeruginosa PAO1 strain to identify potential partners involved in the HptB/HsbR/HsbA pathway. This powerful tool, validated by the interaction previously described between HsbR and HsbA proteins, allowed us to demonstrate that the HsbR response regulator dimerizes through its PP2C/ATPase C-terminal effector domain, an observation further confirmed by pull-down experiments. This will also allow us to identify further new partners in this cascade.

Domaines: Biochimie, Biologie Moléculaire

Laetitia Houot, Adeline Fanni, Sophie De Bentzmann, Christophe Bordi. A bacterial two-hybrid genome fragment library for deciphering regulatory networks of the opportunistic pathogen Pseudomonas aeruginosa.. Microbiology (Reading, Engl.), 2012, 158 (Pt 8), pp.1964--71. ⟨10.1099/mic.0.057059-0⟩. ⟨hal-01458241⟩