Construction of Pseudomonas aeruginosa two-hybrid libraries for high-throughput assays.

Sophie De Bentzmann (1) , Christophe Bordi (1)

1 LISM - Laboratoire d'ingénierie des systèmes macromoléculaires

In Pseudomonas aeruginosa, identification of new partners of a protein of interest could give precious clues to decipher a biological process in which this protein is involved. However, genes encoding for partners of a protein of interest are unknown and frequently scattered throughout the genome. We describe herein the construction and the use of pan-genomic bacterial two-hybrid libraries to identify new partners of a protein of interest encoded by P. aeruginosa.

Domaines: Biochimie, Biologie Moléculaire

Sophie De Bentzmann, Christophe Bordi. Construction of Pseudomonas aeruginosa two-hybrid libraries for high-throughput assays.. Methods in Molecular Biology, 2014, 1149, pp.225--41. ⟨10.1007/978-1-4939-0473-0_19⟩. ⟨hal-01458214⟩